The Bacillus subtilis chromosome region encoding homologues of the Escherichia coli mssA and rpsA gene products

Article Abstract:

A Bacillus subtilis gene codes for a protein, which resembles the Escherichia coli rpsA gene product. One of the domains in the B. subtilis protein binds to two S1 motifs and ribosomes. Northern blot analysis reveals that the rpsA gene is expressed during vegetative growth, at the translational and transcriptional levels in B subtilis. The protein encoded by the ORF before rps homologue resembles the E. coli mssA gene product. Transcription of the mssA gene occurs during sporulation, and the mssA and rpsA genes are separated by a transcription terminator.

Proteins

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The kdgRKAT operon of Bacillus subtilis: detection of the transcript and regulation by the kdgR and ccpA genes

Article Abstract:

Research was conducted to examine the regulation of kdg-operon transcription by the KdgR an CcpA regulatory proteins. The objective was to prove that kdg-operon transcription may be induced by galacturonate and repressed by glucose. To detect the corresponding transcript, northern analysis of total RNA with probes specific to the kdgRKAT region was conducted. Results indicated that knockout mutations in genes ccpA and kdgR remove the effects of glucose and galacturonate on the transcription of the operon, respectively.

Operons

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Organization of the Bacillus subtilis 168 chromosome between kdg and the attachment site of the SPbeta prophage: use of Long Accurate PCR and yeast artificial chromosomes for sequencing

Article Abstract:

The Long Accurate (LA) polymerase chain reaction (PCR) was utilized for the genetic sequencing of Bacillus subtilis 36 kb of the region that is located between the kdg operon and the SPbeta prophage of the lysA-ilvA segment. Sequencing of the 35.5 kb kdg-attSPbeta region indicated the presence of 38 coding sequences and a truncated coding sequence with an ATG start codon. Furthermore, TTG and GTG are the initiation codons for the nine and one coding sequences.

Analysis, DNA, Identification and classification, Polymerase chain reaction, Promoters (Genetics), Genomes, Genetic code, Microbial genetics

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