The ability of Escherichia coli O157:H7 to decrease its intracellular pH and resist the toxicity of acetic acid

Article Abstract:

The effect of acetate on the intracellular pH of Escherichia coli O157:H7 and E. coli K-12 was compared. E. coli O157:H7 produced more D-lactate, obtained a higher glucose consumption rate and accumulated less potassium than K-12. Differences in metabolism and intracellular pH regulation explained the differences in acetate tolerance of 0157:H7. The ability to decrease the intracellular pH and produce large amounts of D-lactate allows E.coli 0157:H7 to decrease changes in pH and to prevent toxic accumulations of intracellular acetate anion.

Composition, Escherichia coli infections, Acetic acid, Acetates, Hydrogen-ion concentration

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Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome-segregation-defective (par) phenotype

Article Abstract:

Isogenic dnaG strains of Escherichia coli with mutant alleles parB and dnaG2903 synthesized DNA at 42 C but still chromosome segregation defective. The dnaG2903 strain converted the single-stranded to double-stranded DNA which indicates that DnaG2903 has retained its primase activity at restrictive temperature. ParB and DnaG2903 could synthesize primer RNA for DNA replication but may be defective in the progression of DNA replication fork which leads to SOS induction.

Phenotype, Phenotypes, Mutation (Biology), Mutation

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