The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway

Article Abstract:

The genes that mediate the metabolism of atrazine in Pseudomonas sp. strain ADP were analyzed by nuclear magnetic resonance (NMR) and mass spectrometry to determine the properties of the atrazine degradation pathway. NMR and high-performance liquid chromatography of the atzA gene indicated the presence of atzB gene which encodes a protein that catalyzes the formation of N-isopropylamelide from hydroxyatrazine. Furthermore, the first and second steps of the atrazine degradation pathway led to the formation of nonphytotoxic and less mobile products.

Analysis, Physiological aspects, Biodegradation, Microbiological synthesis, Herbicides

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Molecular basis of a bacterial consortium: interspecies catabolism of atrazine

Article Abstract:

Research was performed to find out whether Pseudomonas sp. strain ADP contains genes atzA, -B, and -C are present in an atrazine-catabolizing bacterial consortium and how such genes and metabolism are subdivided among member species. Results revealed that different consortium members separately contained the atzA, -B, and -C genes. Furthermore, the consortium metabolized a wider set of triazine ring compounds than did pure cultures in which the atzABC genes had been identified.

Research, Genetic aspects, Bacteria

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Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures

Article Abstract:

Soils exposed to repeated spills of atrazine, alachlor, and metolachlor yield enrichment cultures containing atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) at a concentration of 100 ppm (0.46 mM) as a sole nitrogen source. More than 80% of the S-triazine ring carbon atoms are liberated as CO2-14 when ring-mineralizing activity is restored by mixing pure cultures.

Usage, Soils, Composition

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