The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase

Article Abstract:

Testing whether or not Cbf5p is needed for synthesis of pseudouridine (Psi) in a yeast rRNA showed that after genetic depletion of Cbf5p, formation of Psi in the pre-rRNA is greatly inhibited. That results in accumulation of the unmodified rRNA. Protein A-tagged Cvf5p coprecipitates all tested members of the box H + ACA snoRNAs. It does not coprecipitate box C + D snoRNAs or other RNA species. Cbf5p is likely the rRNA Psi synthase and is an integral part of box H + ACA class snoRNPs. There is much homology in rat nucleolar protein Nap57p, yeast homolog Cbf5p and the Escherichia coli Psi synthase truB/P35.

Yeast fungi, Yeasts (Fungi), Nucleotidases

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A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits

Article Abstract:

Ribosome biogenesis in eukaryotes occurs in a specialized nuclear compartment, the nucleolus, and the newly synthesized subunits must be transported through the nucleoplasm and nuclear pore complexes (NPCs) in order to reach their site of function in the cytoplasm. After an experiment, it is shown that Rrp12p binds to the RNA components of the pre-ribosomes and promotes export of both subunits via its interactions with the nucleoporins and Gsp1p.

Nucleoproteins, Genetic research, Origin of life

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Holoenzyme proteins required for the physiological assembly and activity of telomerase

Article Abstract:

The combined bio-chemical and genetic advantages of Tetrahymena as a telomerase model system is exploited to isolate telomerase-associated proteins and characterize their role in enzyme function. Findings reveal that a role for holoenzyme proteins in determining steady-state telomerase RNA accumulation is universal, even though the mechanism of their interaction with telomerase RNA is not.

Telomerase

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