The log-linear relationship between sexual isolation and sequence divergence in bacillus transformation is robust

Article Abstract:

The relationship between sexual isolation and sequence divergence in bacteria is robust and depends upon factors such as length of donor DNA, concentration of donor DNA and intracellular competition between donor and recipient DNA. Bacteria are more promiscuous than eukaryotes and can exchange genes with organisms that are divergent in DNA sequence, while sexual eukaryotes cannot do so if there is even a two percent variation in DNA sequence. Sexual isolation in bacillus strains in soil may probably depend upon other factors such as donor ability and secretion of DNases.

Analysis, Observations, Microbial genetics

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The size and continuity of DNA segments integrated in Bacillus transformation

Article Abstract:

A restriction-digest approach to examine the size and continuity of DNA segments integrated in Bacillus subtilis transformation is introduced. B. subtilis strain 1A2 was transformed toward rifampicin resistance (coded by rpoB gene) with genomic and polymerase chain reaction-amplified DNA from a given transformant, a recipient and a donor. Restriction analysis revealed that more short DNA fragments were integrated into the chromosome by PCR-amplified segments than by genomic DNA. There was also discontinuity of integration of donor segments within the rpoB gene.

Usage, Bacillus subtilis, Bacterial genetics, Genetic transformation, Restriction enzymes, DNA, DNA restriction enzymes

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The effect of DNA sequence divergence on sexual isolation in Bacillus

Article Abstract:

The quantitative relationship between sexual isolation and DNA sequence divergence in the transformation of a strain of Bacillus was studied. Laboratory strains were transformed and selected for spontaneous mutations which resulted in resistance to rifamycin. Results showed that the relationship fit a log-linear regression formula with a 96% coefficient of determination. There was no significant interaction between sequence divergence and source of DNA. A 93% coefficient of determination was found to exist between sexual isolation and sequence divergence.

Bacterial transformation, DNA sequencers

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