The ribosome-inactivating protein restrictocin deters insect feeding on Aspergillus restrictus

Article Abstract:

The fungal ribosome-inactivating protein, restrictocin, hampers insect feeding on Aspergillus restrictus and may be useful as an insect control agent. An experiment shows that the addition of pure restrictocin to insect diet at 1000 ppm kills 38.5% of Carpophilus freemani (C. freemani) larvae and 62.5% of Spodoptera frugiperda larvae in 48 hrs. However, the protein has no effect on C. freemani adults. C. freemani consume very little phialides with developing spores of Aspergillus restrictus compared to those with Aspergillus nidulans.

Food and nutrition, Plants, Plants (Organisms), Beetles, Diseases and pests

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Polyamines as modulators of microcycle conidiation in Aspergillus flavus

Article Abstract:

The roles of the polyamines (PA) putrescine and spermidine and the PA biosynthetic inhibitors alpha-difluoromethylornithine, alpha-difluoromethylargine, methylglyoxal bis-(guanylhydrazone) and cyclohexylamine on microcycle conidiation (MC) in Aspergillus flavus are determined. Results indicate that diamine and triamine concentrations ranging from O.1 to 5 mM are essential for vegetative growth and are involved in MC. A low diamine-triamine ratio is also essential for spore differentiation in MC.

Growth regulators, Polyamines

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Effect of cytochalasin A on apical growth, actin cytoskeleton organization and enzyme secretion in Aspergillus nidulans

Article Abstract:

An experiment involving Aspergillus nidulans is conducted to examine the role of actin in apical growth and enzyme secretion of the said fungus. This involves the treatment of hyphae with cytochalasin A, a chemical that controls actin polymerization. Results show that normal apical growth, polarized enzyme secretion and hyphal tip shape are achieved by polymerizing actin cytoskeleton.

Actin

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