The virulence plasmid of Yersinia, an antihost genome

Article Abstract:

The Yersinia outer membrane protein (YOP) and the type III virulence system of the pathogenic bacteria were described. This system involved the transfer of bacterial proteins by the extracellular bacteria to the cytosol of the eukaryotic cell. The three species of Yersinia considered as pathogens in mammals are Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Genetic analysis revealed that majority of Yop proteins were necessary for virulence and cytotoxicity activity. The Yops were classified as secreted proteins. The pYV plasmid and the ysc genes encoded the Yop secretion apparatus.

Plasmids, Bacteria, Pathogenic, Pathogenic bacteria, Virulence (Microbiology), Yersinia infections, Bacterial proteins, Yersinia

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Monitoring of Yersinia enterocolitica in murine and bovine feces on the basis of the chromosomally integrated luxAB marker gene

Article Abstract:

A method for monitoring genetically engineered bacteria in the murine and bovine digestive tract was developed. Theprocedure involved the use of luxAB genes, which encodes bacterial luciferase, integrated in the chromosome of Yersinia enterocolitica. The survival of the recombinant bacteria in the digestive tracts of mice and cows was monitored. The results showed that the method is a rapid and reliable system for monitoring genetically engineered bacteria, were easily discriminated from indigenous bacterial populations.

Yersinia enterocolitica

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Simultaneous monitoring of cell number and metabolic activity of specific bacterial populations with a dual gfp-luxAB marker system

Article Abstract:

Research was conducted to examine the dynamics of a specific microbial population wherein both the metabolic activity of the cells and the total bacterial biomass have to be evaluated. The objective was to combine the advantageous characteristics of the two biomarkers, gfp and luxAB, for the simultaneous monitoring of metabolic activity and cell number. Results indicate that the dual marker system is applicable to the quantitation of genetically engineered microorganisms in nature.

Pseudomonas fluorescens, Microbial populations, Recombinant microbial toxins

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