Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters

Article Abstract:

Yeast strains that overproduce threonine in a controlled manner have been derived by placing the HOM3-R2 mutant allele of Saccharomyces cerevisiae under the control of the promoters P(sub GAL1), P(sub CHA1), P(sub CYC1-HSE2) and P(sub GPH1). The amino acid structure of the new yeast strains are then studied under induction and repression conditions. The HOM3 gene of S. cerevisiae codes for aspartate kinase which is vital in regulating the metabolic flux leading to threonine biosynthesis. Threonine is used as a feed additive and its production is mainly through overproductive bacterial strains which yield 80 g of threonine per liter.

Promoters (Genetics), Threonine

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The H+ -ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium accompanying the decrease in intracellular pH and cell viability

Article Abstract:

A study is conducted that examines the pattern of the ATPase activity and the changes in cytosolic pH (pHi) and cell viability in Saccharomyces cerevisiae for the duration of the lag phase needed by the yeast to adjust to the physiological impact of octanoic acid preceding exponential growth. The findings show a considerable heterogeneity of pHi among the cell population used as inoculum. A decline in pHi correlated with the loss of cell viability, as indicated by plate counts, was also observed.

Research, Cell membranes, Plasma membranes, Adenosine triphosphatase, Hydrogen-ion concentration

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Identification of the biosynthetic gene cluster and an additional gene for resistance to the antituberculosis drug capreomycin

Article Abstract:

The cloning and sequence analysis of the Capreomycin (CMN) biosynthetic gene cluster from Saccharothrix mutabilis subsp. capreolus ATCC 23892 is discussed. Results suggest that the transfer of gene cluster into S. lividans 1326 leads to heterologous production of the CMN antituberculosis drug

Analysis, Bioengineering

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