Transcripts of the genes sacB, amyE, sacC and csn expressed in Bacillus subtilis under the control of the 5' untranslated sacR region display different stabilities that can be modulated

Article Abstract:

Transcripts of the genes sacB, amyE, sacC and csn expressed in Bacillus subtilis are discussed. They are under the control of the 5' untranslated sacR region and have been found to display different stabilities that can be modulated. The mRNA steady-state level might be controlled by mRNA decay and/or translation mechanisms might be adapted to a certain growth phase. To test the hypothesis, the transcriptional efficiency of the gene fusions and the stability of the resulting transcripts were studied. Chitosanase was added to the study.

Bacterial genetics, Microbial enzymes, Genetic transcription, Transcription (Genetics), Messenger RNA, Extracellular matrix, Microbiological chemistry

---

yveB, encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis

Article Abstract:

Research has been conducted on the transcription of the clustered genes on the Bacillus subtilis chromosome. The results indicate that sacB which encodes levansucrase is a proximal sucrose-inducible operon gene.

Analysis, Gene expression, Operons, Microbiological research, Sucrose

---

Kinetics of the secretion of Bacillus subtilis levanase overproduced during the exponential phase of growth

Article Abstract:

The Bacillus subtilis levanase structural gene sacC was expressed under the regulated control of sacR, the inducible levansucrase leader region, in a degU32(Hy) strain. Comparison of the findings with those previously observed with levansucrase and alpha-amylase overproduced in the same genetic and physiological situation, indicated that the precursor processing is more efficient in levanase and alpha-amylase than in levansucrase.

Protein folding

Similar abstracts:

• Abstracts: Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWWO under various conditions of nutrient limitation in chemostat culture
• Abstracts: Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD
• Abstracts: Characerization of the expression and activity of the periplasmic nitrate reductase of Paracoccus Pantotrophus in chemostat cultures
• Abstracts: Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods
• Abstracts: Sequence and expression analysis of the ompA gene of Rickettsia peacockii, an endosymbiont of the Rocky Mountain Wood Tick, Dermacentor andersoni

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.