Transfer of IncP plasmids to extremely acidophilic Thiobacillus thiooxidans

Article Abstract:

The transfer of broad-host range IncP plasmids to the extremely acidophilic Thiobacillus thiooxidans from Escherichia coli was discussed. The plasmids, which include RP4, R68.45, RP1::Tn501 and PuB307, were directly transferred by conjugation at frequecies of 10 (sub -15) to 10 (sub -17) per recipient. In addition T. thioxidans showed the ability to transfer back the plasmid RP4 to E. coli at a frequency of 10 (sub -3). The study also shoed that kanamycin and tetracycline resistance markers were expressed in T. thioxidans.

Genetic aspects, Plasmids, Microbial genetic engineering, Genetic markers

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Growth kinetics of Thiobacillus thiooxidans on the surface of elemental sulfur

Article Abstract:

The rate at which Thibacillus thiooxidans grows on the surface of elemental sulfur may depend upon the concentration of adsorbed cells and the proportion of unnoccupied sites on the surface. As the sulfur is oxidized to sulfate, the concentration of adsorbed and free floating cells increase, but the adsorbed cell concentration approaches a maximum level. Growth rates and kinetic proportions of adsorption are estimated.

Observations, Bacterial growth

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Kinetic constant variability in bacterial oxidation of elemental sulfur

Article Abstract:

The kinetic constant variability in the bacterial oxidation of elemental sulfur for different batch cultures is being explained.

Physiological aspects, Sulfur, Sulphur, Sulphur bacteria

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