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Transformation of 2,4,6-trinitrotoluene by Pseudomonas pseudoalcaligenes JS52

Article Abstract:

Nitrobenzene-grown cells of Pseudomonas pseudoalcaligenes strain JS52 rapidly degraded 2,4,6-trinitrotoluene (TNT) from culture fluids via a novel pathway involving enzymes from nitrobenzene degradation. Cells and cell extracts of Pseudomonas pseudoalcaligenes strain JS52 also convert TNT to end products via hydroxylamino intermediates such as 2,4-dihydroxylamino-6-nitrotoluene (DHANT). Furthermore, the conversion of TNT to DHANT was catalyzed by nitrobenzene nitroreductase.

Author: Spain, Jim C., Fiorella, Paul D.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Microbiological synthesis, Pseudomonas

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Biodegradation of 3-Nitrotyrosine by Burkholderia sp. strain JS165 and Variovorax paradoxus JS171

Article Abstract:

Variovarux paradoxus JS171 and Burkholderia sp. strain JS165 were isolated from soil slurries when 3-nitrostyrosine was provided as the sole carbon, nitrogen and energy source. The cascade of reactive nitrogen species generated from nitric oxide, which causes modification of proteins, lipids and nucleic acids in a wide range of organisms, shows that there is a significant flux of 3-nitrotyrosine in such environments.

Author: Nishino, Shirley F., Spain, Jim C.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
Science & research, Biological Product (except Diagnostic) Manufacturing, Drugs, Nucleic Acids, Soil research

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Oxidative transformation of aminodinitrotoluene isomers by multicomponent dioxygenases

Article Abstract:

Three bacterial dioxygenases can hydroxylate the TNT ring if one of the nitro groups is reduced to the amine. This increases the electron density of the ring and makes it more susceptible to attack. These bacteria could be used in the biodegradation of TNT.

Author: Spain, Jim C., Smets, Barth F., Johnson, Glenn R.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001

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Subjects list: Research, Biodegradation, Microbial enzymes
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