Induced natural transformation of Acinetobcter calcoaceticus in soil microcosms

Article Abstract:

A study was conducted on the factors that influence Acinetobacter calcoaceticus BD413 natural transformation in slit loam soil microcosms. The application of salts, freshly-added DNA and a simple carbon source resulted in induced transformation. Results demonstrate the transformability of Acinetobacter calcoaceticus with chromosomal DNA within hours after activation by soil nutrients.

Bacterial transformation, Extrachromosomal DNA

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Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA

Article Abstract:

Research was conducted under optimized laboratory conditions to examine the transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA based on homologous recombination. Plasmid pFG4 delta-nptII was constructed and introduced in Acinetobacter sp strain BD413 to facilitate the stable integration of transgenic plant DNA. Results demonstrate that some transformants obtained additional DNA in addition to restoring the 317-bp deletion.

Genetic aspects, Plant genetics, Genetically modified plants, Sugar beets, Sugar beet

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