Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli

Article Abstract:

The unique properties of plasmid pJB658 are utilized to express a host-toxic antibody fragment, scFv-phOx, in high-cell-density cultivation of Escherichia coli. The findings indicate that soluble hapten 2-phenyloxazol-5-one could be produced to a high volumetric yield in high-cell-density cultures of Escherichia coli.

Plasmids, Host-bacteria relationships

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Identification of common subpopulations of non-sorbitol-fermenting beta-glucuronidase-negative Escherichia coli 0157:H7 from bovine production environments and human clinical samples

Article Abstract:

Incorporation of six markers into a multilocus genotyping assay, termed lineage-specific polymorphism assay-6 (LSPA-6) is described. Analysis of over 1,400 0157:H7 strains with the LSPA-6 demonstrated that five genotypes comprise over 91% of the strains, suggesting that these subpopulations may be widespread.

Genotype, Genotypes, Genetic polymorphisms

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