Two-component transcriptional regulation of N-acyl-homoserine lactone production in Pseudomonas aureofaciens

Article Abstract:

Bacterial populations require precise gene expression regulation as a means of responding to changing environmental conditions. Two-component signal transduction systems facilitate bacterial gene expression regulation. Both systems are composed of a sensor kinase and a cytoplasmic response regulator. GacS/GacA are also present and serve as controllers of phenazine production in an AHL-independent environment. Results of the study were based on AHL preparations derived from 5-ml cultures of Pseudomonas strains that were grown overnight at 28 deg Celcius with shaking in PPMD broths.

Growth, Bacteria, Macrolide antibiotics, Macrolides, Lactones

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Validation of microcosms for examining the survival of Pseudomonas aureofaciens (lacZY) in soil

Article Abstract:

An intact soil core appears to accurately predict the survival of recombinant microorganisms in the environment. Pseudomonas aureofaciens (lacZY) inoculated into 5 by 15 cm polyvinyl chloride cores containing either bulk or rhizosphere soil exhibited survival similar to that seen in the field. Inoculum in 50 g of dried and sieved (disturbed) soil did not accurately predict survival. The simple, cheap intact core may provide a standard environmental microcosm for risk assessment of recombinant microbes.

Models, Risk assessment, Environmental aspects, Soil research

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Fate of the biological control agent Pseudomonas aureofaciens TX-1 after application to turfgrass

Article Abstract:

Applying the biological control agent Pseudomonas aureofaciens TX-1 to turfgrass does not substantially affect the indigenous microbial population. This bacterium produces the antimicrobial compound phenazine-1-carboxylic acid and is used to control soil fungi.

Fungi, Biological control, Antitoxins

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