Use of a fluorescent redox probe for direct visualizationn of actively respiring bacteria

Article Abstract:

A new method for detecting activelyrespiring bacteria was developed. The procedure involved the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), which is reduced by the electron transport chain to a fluorescent formazan which accumulates intracellularly, and can be detected by epifluorescent microscopy. The procedure was used to analyze samples of municipal wastewater, groundwater and seawater. The results showed that CTC counts were generally lower than total counts, but comparable to standard aerobic plate counts. However, the use of CTC greatly facilitated detection and enumeration of respiring bacteria.

Identification and classification, Bacteria, Aerobic, Aerobic bacteria, Microbial respiration

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Agar underlay method for recovery of sublethally heat-injured bacteria

Article Abstract:

A method, called the agar underlay method, for culture and recovery of sublethally heat-injured bacteria has been developed. The method has advantages vs other injury recovery methods. Unlike agar overlay methods, the underlay method allows the typical selective-medium colony morphology to develop for food-borne pathogens that are heat-injured, for instance Escherichia coli O157:H7 and Salmonellla typhimurium. Colonies can be more easily picked for further characterization.

United States, Statistical Data Included, Usage, Diagnosis, Environmental aspects, Escherichia coli, Foodborne diseases, Salmonella, Cell culture, Tissue culture, Agar

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Estimation of the state of the bacterial cell wall by fluorescent in situ hybridization

Article Abstract:

Fluorescent in situ hybridization (FISH) was used assessing the state of the bacterial cell walls of the gram-positive bacterium Lactococcus lactis. FISH was utilized in studying the effect of phage infection on the capacity of L. lactis to hybridize a general HRP-labeled 16S RNA-targeted probe. The obtained results demonstrate that FISH used in conjunction with HRP-labeled probes is an excellent method in studying the cell walls of gram-positive bacteria.

Physiological aspects, Bacteria, Bacterial cell walls, Fluorescent antibody technique

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