Use of a tetracycline-inducible system for conditional expression in Mycobacterium tuberculosis and Mycobacterium smegmatis

Article Abstract:

The use of a tetracycline-inducible expression system in Mycobacterium tuberculosis and Mycobacterium smegmatis was studied. When a reporter gene was used, it showed that tetracycline -inducible expression occurred in M. smegmatis and M. tuberculosis and that expression levels were titrable to some extent by varying the concentration of tetracycline.

Genetic aspects, Mycobacterium tuberculosis, Gene expression

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Tetracycline-dependent conditional gene knockout in Bacillus subtilis

Article Abstract:

Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. The implementation of both approaches with full regulatory range in gram-positive bacteria as exemplified in Bacillus Subtilis is reported.

Bacillus subtilis, Genetic regulation

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Antimicrobial susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus isolates from Louisiana Gulf and retail raw oysters

Article Abstract:

The antimicrobial susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus isolates recovered from Louisiana Gulf and retail oysters are examined. Vibrio parahaemolyticus has exhibited higher MICs for cefotaxime, ciprofloxacin and tetracycline than Vibrio vulnificus.

Shellfish, Shellfish Fishing, Oysters, Drug resistance in microorganisms, Microbial drug resistance, Vibrio, Cefotaxime, Crassostrea

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