Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters

Article Abstract:

An oligonucleotide probe, designated VP5 and derived from the hemolysin gene, was developed to detect for the presence of Vibrio parahaemolyticus in shellfish. Tests on artificially contaminated oysters showed that the probe could detect V. parahaemolytucus at levels of about 10(super 6) colony forming units. DNA hybridization tests confirmed the probe's specificity to V. parahaemolyticus, although it did not hybridize to DNA of five V. parahaemolyticus isolates. These results demonstrate that VP5 may be used to directly identify V. parahaemolyticus in food samples.

Evaluation, Identification and classification, DNA probes, Vibrio

---

Detection of hepatitis A virus RNA in oyster meat

Article Abstract:

Low concentrations of viruses in shellfish can be detected through nucleic acid amplification by PCR (RT-PCR). Hepatitis A virus (HAV) was detected in oyster meat by reverse transcription-PCR. A technique for identifying HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation or injection, was developed. Using dot blot hybridization detection of amplicons from the RT-PCR, fast screening of a huge number of samples is possible.

Analysis, Hepatitis A virus, Hepatovirus

---

Magnification of tributyl tin toxicity to oyster larvae by bioconcentration in biofilms of Shewanella colwelliana

Article Abstract:

A study was conducted on the influence of dissolved and bioconcentrated tributyl tin on oyster larvae. Shewanella colwelliana biofilm bioconcentration was observed to stem the metamorphosis and attachment of oyster larvae on bottom surfaces. Tributyl tin concentrations less than 5ng/ml exhibit no lasting effect on larval activity. Results have potential applications in environmental heavy metal toxicity studies.

Research, Tributyltin, Larvae, Tributylin

Similar abstracts:

• Abstracts: Use of bioluminescence for detection of genetically engineered microorganisms released into the environment. Gene copy number polymorphisms in an arbuscular mycorrhizal fungal population
• Abstracts: Biotic and abiotic factors affecting plasmid transfer in Escherichia coli. Nitroaromatics are substrates for the TOL plasmid upper-pathway enzymes
• Abstracts: Lack of virulence factors in Eschericia coli strains of enteropathogenic serogroups isolated from water. Comaparison of Campylobacter isolates from poultry and humans: association between in vitro virulence properties, biotypes, and pulsed-field gel electrophoresis clusters
• Abstracts: Occurence of tributyltin-tolerant bacteria in tributyltin- or cadmium-containing seawater. Association of luminous bacteria with artificial and natural surfaces in Arabian Gulf seawater
• Abstracts: Occurence of tributyltin-tolerant bacteria in tributyltin- or cadmium-containing seawater. part 2 Occurence of fumonisin in forage grass in New Zealand

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.