Use of cosmid-derived and chromosome-specific canine microsatellites

Article Abstract:

A study was conducted to determine a set of microsatellite-containing cosmid clones covering each chromosome that can be mapped by fluorescence in situ hybridization and genetically mapped by genotyping microsatellites. Results indicated that the development of microsatellite-containing cosmid clones significantly enhances the quality and use of canine genome maps. In addition, the use of chromosome-specific libraries to maximize the density of markers is critical in filling in gaps.

Methods, Animals, Hybridization

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Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies

Article Abstract:

Research was conducted to analyze the use of random amplified polymorphic DNA (RAPD) assay to genetic studies of dogs and examine the amplification of RAPD sequences with primer pairs. Polymerase chain reactions were carried out using the GeneAmp PCR System 9600. Results indicated that a novel polymorphism in the restriction digest products. Findings also suggested that RAPD can be effectively utilized as molecular markers in genetic studies of diseases in dogs.

Diseases, DNA, Genetic polymorphisms

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FISH mapping and identification of canine chromosomes

Article Abstract:

A study was conducted to analyze the fluorescence in situ hybridization mapping and identification of canine chromosomes. Chromosome preparations were determined by mitogenic simulation of canine peripheral lymphocytes. Canine metaphase preparations were then obtained by phytohemagglutinin stimulation of peripheral lymphocytes. Results indicated that the development of chromosome paints for the canine karyotype is an important tool in chromosome identification.

Genomes

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