Use of gel retardation to analyze protein-nucleic acid interactions

Article Abstract:

The formation of protein-nucleic complexes was evaluated using gel retardation or band shift technique. Gel retardation was the method of choice because of its high analytical power and technical simplicity. The reduced electrophoretic mobilty of the nucleic acid component in a gel matrix may give a simplified understanding of the physical behavior of protein-nucleic acid complexes in gels. The factors affecting the complex's migration were gel pore size, the ratio of mass of protein to that of nucleic acid, and binding induced conformational changes. The complex's stability was found to be influenced by the composition and ionic strength of the gel buffer.

Usage, Gel electrophoresis, Proteins, Nucleic acids, Protein conformation

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Meiotic instability of Pythium sylvaticum as demonstrated by inheritance of nuclear markers and karyotype analysis

Article Abstract:

A study of the inheritance of RFLP and random amplified polymorphic DNA (RAPD) markers by pulsed field gel electrophoresis of progeny from a sexual outcross of opposite mating types of Pythium sylvaticum reveals the presence of various electrophoretic karyotypes and minimum number of chromosome-sized DNAs in parental oogonial and antheridial isolates. The summation of band sizes in each-isolate and the minimum number of chromosome-sized DNAs are different among progeny karyotypes. Southern analysis reveals that karyotypic polymorphisms result from aneuploidy and translocations and length mutations.

Analysis, Karyotypes, Meiosis

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Two-way chemical signaling in Agrobacterium-plant interactions

Article Abstract:

The molecular aspect of chemical signalling between plants and Agrobacterium species is investigated. Agrobacterium can detect low-molecular-weight compounds which dissipate from plant wounds. These include phenolic compounds, monosaccharides in acidic environments, acidic polysaccharides and opines. Agrobacterium strains, in return, discharge chemicals to the plant, such as pectinase, phytohormones and deoxyribose nucleic acid. The relationship gives new insight into the chemical nature of phytopathogenic infection.

Plant-pathogen relationships, Molecular biology

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