Use of green fluorescent protein (GFP) to study the invsion pathways of Edwardsiella tarda in in vivo and in vitro fish models

Article Abstract:

Results demonstrate that fluorescent proteins are a useful tool for visualizing bacteria in the infected host tissues as illustrated by studying invasion pathways of a fish pathogen. The bacterial infection causes edwardsiellosis disease reported in eels, salmons, cat fish and others.

Usage, Infection, Host-bacteria relationships, Fluorescence microscopy, Epithelial cells, Bacteria, Pathogenic, Pathogenic bacteria

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Edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity

Article Abstract:

Edwardsiella tarda mutants have been found to be defective in siderophore production, motility, catalase activity and serum resistance, factors which may correlate with virulence in fish. It may be that disrupting genes involved in these things could attenuate the pathogen and impair ability to infect fish. Ed. tarda is a Gram-negative bacterium that causes a systemic fish infection, edwandsiellosis. Virulence factors and genetic determinants have now been studied by using TnphoA transposon mutagenesis to set up a library of 440 alkaline phosphatase fusion mutants from 400,000 transconjugants from Ed. tarda PPD 130/91.

Gene mutations, Gene mutation, Environmental aspects, Genetic aspects, Gram-negative bacteria, Fisheries, Microbial enzymes, Cytochemistry, Virulence (Microbiology)

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Internalization and cytotoxicity are important virulence mechanisms in vibrio-fish epithelial cell interactions

Article Abstract:

Research was conducted to examine the interaction between fish epithelial cells and the Vibrio species. To elucidate the intimate vibrio-host relationship, phase-contrast, confocal and scanning electron microscopy were used. Inhibitors that interrupt cytoskeleton or signal transduction pathways were also used to analyze the internalization process of 24 vibrio strains. Results demonstrated that cytopathology induced by vibrios in both grunt-fin tissue and epithelioma papillosum of carp cells was an indicator of Vibrio virulence.

Cell-mediated cytotoxicity, Cell mediated cytotoxicity, Vibrio, Host-parasite relationships

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