Use of homoduplex ribosomal DNA spacer amplification products and heteroduplex cross-hybridization products in the identification of Salmonella serovars

Article Abstract:

Homoduplex and heteroduplex products obtained through the ribosomal DNA (rDNA) spacer amplification method can be effectively used for identifying Salmonella serovars. Amplification of the homoduplex and heteroduplex regions of prokaryotic rDNA generates homoduplex double-stranded DNA structures and heteroduplex single-stranded DNA structures. These structures result in product patterns of ribosomal spacer and heteroduplex polymorphism types. Their sensitivity to intergenic spacer sequence composition serves in differentiating Salmonella serovars.

Methods, Salmonella, Recombinant DNA, Gene amplification

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Rapid interaction of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms

Article Abstract:

The amplification of spacer regions between 16S and 23S genes of the rRNA genetic loci by a unified set of primers and polymerase chain reaction (PCR) conditions is discussed. Prior to amplification, selection of the generic pair of priming sequences were conducted. Electrophoresis was used to resolve the spacer amplification products. The utility of PCR amplification for bacterial identification was demonstrated.

Usage, Polymerase chain reaction

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Modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum and its application to clinical and epidemiological investigation

Article Abstract:

Researchers describe a rapid method for genotyping Cryptosporidium parvum from clinical and environmental samples and used it to investigate an outbreak of cryptosporidiosis in schoolchildren who had visited a farm. The method is based on a nested PCR-RFLP technique that identifies polymorphisms of the bacterial gene for thrombospondin-related adhesive protein.

Genetic aspects, Coccidia, Cryptosporidium

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