Variation of electrophoretic karyotypes in genetically different strains of Saccharomycopsis fibuligera and Yarrowia lipolytica

Article Abstract:

A study of the variation of electrophoretic karyotypes in genetically different Saccharomycopsis fibuligera and Yarrowia lipolytica strains by analyzing auxotrophic mutants obtained from the intergeneric hybrid N14i60 met reveals morphological similarities between the mutants and N14i60. Analysis of the DNA banding patterns of the S. fibuligera and Y. lipolytica strains indicates several resemblances and variations, and identified four different types, according to their specific locations in the pedigree chart.

Research, Yeast, Yeast (Food product), Variation (Biology), Microbial mutation

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Fluorescent oligonucleotide rDNA probes that specifically bind to a common nanoflagellate, Paraphysomonas vestita

Article Abstract:

A series of fluorescent oligonucleotide probes that can hybridize with unique sequences of the small subunit (SSU) ribosomal RNA (rRNA) of Paraphysomonas vestita were prepared for bacterial identification. The fluorescent oligonucleotide probes specifically bonded to Paraphysomonas vestita and imparted a strong fluorescence to the nanoflagellate. Furthermore, SSU rRNA gene of the nanoflagellate exhibited numerous sequence differences compared to other Paraphysomonas species.

Methods, Nucleic acid probes, Ribosomal RNA, Microbial genetics

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Characterization, localization and functional analysis of Gpr1p, a protein affecting sensitivity to acetic acid in the yeast Yarrowia lipolytica

Article Abstract:

Results demonstrate that the expression of the GPR1 gene, encoding Gpr1p protein, is stimulated by acetic acid. However, the GPR1 gene is not essential for utilizing acetic acid as a carbon source during yeast's growth. Data show that Gpr1p is an integral membrane protein and is confined to the plasma membrane.

Austria, Germany, All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Acetic Acid, Physiological aspects, Growth, Membrane proteins, Gene expression, Yeast fungi, Yeasts (Fungi), Company growth

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