Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans

Article Abstract:

The green fluorescent protein (GFP) of the jellyfish Aequorea victoria is used as a marker for gene expression and protein localization in Candida albicans. There was no fluorescence detected when either the wild type(GFP) gene or the mutated GFP gene in which the CTG codon has been changed to TTG was expressed in Candida albicans. A synthetic GFP gene with optimal codons for translation as well as two chromophore mutations that increase GFP fluorescence is functional. This yeast-enhanced GFP gene generates fluorescence in C. albicans.

Analysis, Fluorescence, Candida albicans

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Endless possibilities: translation termination and stop codon recognition

Article Abstract:

How stop codons are reassigned to encode sense at a certain frequency in various RNAs is discussed in this review article. The discussion is based on current understanding of termination and of stop codon recognition mechanisms. Use of optimized complex reconstituted in vitro termination reactions to find the roles of key termination factors has been helpful. The solution of tertiary structures of termination factors has made possible reappraisal of termination factor structurefunction.

United Kingdom, Statistical Data Included, Physiological aspects, Viral research, Proteins, Virus research, Transfer RNA, Genetic translation, Translation (Genetics)

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Codon usage in the Mycobacterium tuberculosis complex

Article Abstract:

The application of alternative synonymous codons in Mycobacterium tuberculosis and M. bovis genes were studied. Putative optimal codons were determined for 15 amino acids. The degree of bias towards optimal codons in an M. tuberculosis gene was correlated with that in homologues from Escherichia coli and Bacillus subtilis. The set of selectively favored codons appeared to be highly conserved between M. tuberculosis and Cornyebacterium glutamicum.

Mycobacterium tuberculosis, Chemical evolution, Molecular evolution, Mycobacterium bovis

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