mRNA stabilization by poly(A) binding protein is independent of poly(A) and requires translation

Article Abstract:

The stabilization of mRNA by poly(A) binding protein occurs independently of poly(A) and must involve translation. mRNA stability and translation are helped by presence of a poly(A) tail. The tail, in vivo, interacts with a conserved polypeptide, the poly(A) binding protein (Pab1p). Pab1p has been connected into the 3' untranslated region (UTR) of reporter mRNAs by connecting it to MS2 coat protein and putting MS2 binding sites in the reporter 3' UTR. It is then possible to separate Pab1p function from RNA binding activity it has. mRNAs with no poly(A) tail are stabilized in vivo by Pab1p. Parts of Pab1p necessary for stabilization are genetically unique when compared to those necessary for poly(A) binding. The main or only function of poly(A) relative to mRNA stability is to bring Pab1p to mRNA.

Protein binding, Messenger RNA, Genetic translation, Translation (Genetics)

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Specific telomerase RNA residues distant from the template are essential for telomerase function

Article Abstract:

Certain telomerase RNA residues far from the template are required for telomerase function. Reverse transcriptase (RT) telomerase adds telomeric repeats to chromosome ends through using as a template a sequence inside its endogenous RNA component. Activities of nontemplate telomerase residues in the telomerase RNA of Kluyveromyces lactic, a yeast, at the budding stage, have been studied. About half of the RNA residues were not needed for function, but four regions were required in vivo for telomerase activity.

Analysis, Telomeres, Yeast, Yeast (Food product), Cellular control mechanisms, Cell regulation

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Developmentally programmed assembly of higher order telomerase complexes with distinct biochemical and structural properties

Article Abstract:

In Euplotes crassus, a ciliate, telomerase controls telomere maintenance in the vegetative growth period and de novo telomere synthesis in macronuclear development. Telomerase in the vegetative stage of the life cycle is a 280-kD complex. It can put telomeric repeats onto telomeric DNA primers only. There is evidence of the existence of developmentally controlled higher order telomerase complexes with distinct structural and biochemical properties.

DNA repair, Protozoa, Protozoans, Ciliata, Ciliates

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