Short hairpin RNA-expressing bacteria elicit RNA interference in mammals

Article Abstract:

A demonstration that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in vitro and in vivo is presented. Nonpathogenic Escherichia coli were engineered to transcribe the listeriolysin O gene HlyA, which encode two bacterial factors needed for successful transfer of the shRNAs into mammalian cells.

Analysis, Genetic transformation, RNA interference

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Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus

Article Abstract:

A modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli is developed. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC and a transcriptional terminator from fliC, into the gene encoding the polypeptide of interest.

Secretion, Polypeptides

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Gene patents and genetic testing in the United States

Article Abstract:

The impact of the introduction of genetic testing into mainstream medicine on the healthcare system in the United States is discussed. The findings show that the gene patents impose restrictions on such practices and hence can affect the healthcare system adversely.

Forecasts, trends, outlooks, Patents & copyrights, Forecasts and trends, Market trend/market analysis, Intellectual property, Genetic screening, Genetic testing, Human genome

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