Stable expression of shRNAs in human CD34(super +) progenitor cells can avoid induction of interferon responses to siRNAs in vitro

Article Abstract:

The immunostimulatory effects of lipid-delivered small interfering RNAs (siRNAs) versus Pol III promoter-expressed small hairpin RNAs (shRNAs) in primary CD34(super +) progenitor-delivered hematopoietic cells are investigated. The results have shown that in this system, lipid-delivered siRNAs are potent inducers of interferon (IFN) alpha (IFNalpha) and type I IFN gene expression, whereas the same sequences when expressed endogenously are nonimmunostimulatory.

Biological products exc. diagnostic, Interferon, RNA interference

---

Sorting out metagenomes

Article Abstract:

An innovative screen that rapidly sorts thousands of environmental DNA clones to identify those with a desired metabolic activity is presented with the help of a designed high-throughput screen for catabolic pathways, substrate-induced gene expression screening' or 'SIGEX'. The SIGEX template allows infinite variation in the functions to be discovered, the regulator molecules to be employed and the reporter proteins to be detected.

Identification and classification, Protein research

---

Laying hold on eternal life?

Article Abstract:

Researchers studied cultures of pig fetal fibroblasts to establish the relationship of cloning between aging and telomere biology.

Similar abstracts:

• Abstracts: Pantene Pro-V Brunette Expressions Shampoo; Conditioner. Pantene Pro-V Blonde Expressions Shampoo; Conditioner
• Abstracts: The patentability of human embryonic stem cells in Europe. FDA likely to further restrict erythropoietin use for cancer patients

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.