2,2,2-triflouroethanol-induced molten globule state of concanavalin A and energetics of 8-anilinonaphthalene sulfonate binding: Calorimetric and spectroscopic investigation

Article Abstract:

The interactions of 2,2,2-triflouroethanol (TFE) with concanavalin A are investigated using a combination of differential scanning calorimetry, isothermal titration calorimetry (ITC), circular dichroism (CD) and fluorescence spectroscopy at pH 2.5 and 5.2. The ITC results indicate that the binding of 8-anilinonaphthalene sulfonate (ANS) to the molten globule state and acid-induced state displays heterogeneity with two classes of non-interacting binding sites.

Industrial organic chemicals, not elsewhere classified, Ethanol, Ethyl Alcohol Manufacturing, Analysis, Alcohol, Volumetric analysis, Fluorescence spectroscopy, Alcohol, Denatured

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Binding of naproxen and amitriptyline to bovine serum albumin: Biophysical aspects

Article Abstract:

Binding of the drugs naproxen and amitriptyline to bovine serum albumin (BSA) is studied using isothermal titration calorimetry (ITC), in combination with fluorescence and circular dichroism spectroscopies. The results have provided a quantitative understanding of the binding of naproxen and amitriptyline to BSA, which is important in understanding their effect as therapeutic agents individually and in combination therapy.

Biological Product (except Diagnostic) Manufacturing, India, Biological products exc. diagnostic, Blood Albumin, Serum albumin, Atomic properties, Naproxen, Amitriptyline

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Binding of 8-anilinonaphthalene sulfonate to dimeric and tetrameric concanavalin A: Energetics and its implications on saccharide binding studied by isothermal titration calorimetry and spectroscopy

Article Abstract:

The binding of 8-anilinonaphthalene sulfonate to concanavalin A was investigated by isothermal titration calorimetry (ITC) and circular dichroism studies. Enhancement of the binding constants and fluorescence intensity from native to pH 2.5 suggests that ANS binding is influenced by the protein charge and by the favorable alteration in the structure of concanavalin A as suggested also by near-UV CD spectra.

Science & research, Industrial Organic Chemicals, Cyclic Crude and Intermediate Manufacturing, Sulfolane, Research, Spectra, Circular dichroism, Sulfones

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