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Characterization of low-energy chlorophylls in the PSI-LHCI supercomplex from Chlamydomonas reinhardtii. A site-selective fluorescence study

Article Abstract:

Site-selective fluorescence spectroscopy is used to characterize spectral properties of low-energy chlorophylls (Chls) in high-quality photosystem I (PSI)-LHCI preparations from the green alga Chlamydomonas reinhardtii. A simple technique offers an access to parameters of the red Chl pool, such as absorption maximum, Stokes' shift, and ratio of homogeneous and inhomogeneous broadening, provides some conclusions on the electron-phonon coupling of the red Chls in this system.

Author: Dekker, Jan P., Ihalainen, Janne A., van Grondelle, Rienk, Gibasiewicz, Krzysztof, Szrajner, Anna, Germano, Marta
Publisher: American Chemical Society
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2005
Science & research, Poland, Photosynthesis research, Chlamydomonas

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Fluorescence decay kinetics of wild type and D2-H117N mutant photosystem II reaction centers isolated from Chlamydomonas reinhardtii

Article Abstract:

Time-resolved fluorescence spectroscopy has been used to investigate energy transfer in wild type and D2-H117N mutant PS II reaction centres isolated from Chlamydomonas reinhardtii. It is shown that the D2-H117N reaction centre is a conservative mutant, replacing the histidine at site 117 with asparagine.

Author: Johnston, Heather G., Wang, Jun, Ruffle, Stuart V., Sayre, Richard T., Gustafson, Terry L.
Publisher: American Chemical Society
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2000
Protein binding

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Substitution of chlorophyll into the inactive branch pheophytin-binding site impairs charge separation in photosystem II

Article Abstract:

The PSII RC cofactor symmetry is altered by replacing the inactive-branch of pheophytin with chlorophyll. The D1-L210 residue is mutated to a histidine (D1-L21OH) to provide a Mg ligand for Chlorophyll.

Author: Wasielewski, Michael R., Sayre, Richard T., Hemann, Craig, Xiong, Ling, Seibert, Michael, Gusev, Alexey V., Hille, C. Russ
Publisher: American Chemical Society
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
Histidine, Magnesium, Magnesium (Metal), Chemical properties

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Subjects list: Research, Chlorophyll
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