Discrimination between single Escherichia coli cells using time-resolved confocal spectroscopy

Article Abstract:

A technique for rapidly discriminating between single-cell populations within a flowing microfluidic stream is reported. Single-cell time-correlated single-photon counting (scTCSPC) and photon burst spectroscopy are employed to characterize individual Escherichia coli cells expressed within either green, cyano, or yellow fluorescent protein and the calculated burst width characteristics are found to be largely controlled by the fluorescence quantum yield and adsorption cross section of the proteins used.

Physiological aspects, Escherichia coli, Bacterial proteins, Adsorption, Chemical properties

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The "Le Chatelier's principle'-governed response of actin filaments to osmotic stress

Article Abstract:

The mechanism of the inhibitory effect of F-actin on osmotic stress-induced water flow is elucidated on the basis of nonequilibrium thermodynamics, addressing a novel problem of how osmotic stress changes the chemical potential of the F-actin. F-actin is shown to have an inhibitory effect on the osmotic stress-driven water flow and this is the first report showing that the Le Chatelier's principle applies to the reaction of biopolymers against equilibrium disturbances such as osmotic stress.

Osmotic pressure, Actin, Biopolymers, Thermal properties, Chemical equilibrium, Properties, Structure

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A Lattice-Gas Cellular Automaton to model diffusion in restricted geometries

Article Abstract:

A new and general Lattice-Gas Cellular Automata (LGCA) consisting in a d-dimensional network of hypercubically arranged cells is developed. The results of the application of the model to the specific problem of geometrical restricted long-range diffusion are utilized to interpret the behaviors of particles in tight confinement.

Lattice theory

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