Excitation decay pathways of Lhca proteins: A time-resolved fluorescence study

Article Abstract:

The room-temperature fluorescence decay properties of four monomeric Lhca proteins (Lhca1-4) and a dimeric Lhca1/4 protein are studied using a Streak camera setup with about 5 ps time resolution. This study shows that Lhca proteins have faster decay lifetimes and lower emission yields compared to their counterparts of photosystem II (PSII) antenna proteins, light-harvesting complex II (LHCII) and CP29.

France, Analysis, Excitation (Physiology)

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Ultrafast polarized fluorescence measurements on monomeric and self-associated melittin

Article Abstract:

The anisotropic and magic-angle fluorescence decay of the single tryptophan (Trp) residue of melittin, a bee venom peptide, is investigated by time-resolved fluorescence anisotropy using a streak camera setup. Decay-associated spectra of the isotropic fluorescence decay show three time components in the range of ~20 picoseconds, 500 picoseconds, and ~3500 picoseconds, respectively.

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Ultrafast polarized fluorescence measurements on tryptophan and a tryptophan-containing peptide

Article Abstract:

The photophysics of Trp (tryptophan) and Trp in a small 22-mer peptide is investigated using polarized fluorescence spectroscopy with picosecond time resolution. Both Trp alone and Trp in the peptide exhibit a multiexponential isotropic fluorescence decay that is associated with ground-state heterogeneity due to different rotamers of Trp.

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