Function of membrane protein in silica nanopores: Incorporation of photosynthetic light-harvesting protein LH2 into FSM

Article Abstract:

The folded-sheet silica mesoporous material (FSM) having nanometer-size pores inside was filled with a high amount of functional membrane protein complex, namely the photosynthetic light-harvesting complex LH2 to capture the light energy. The LH2 complex in the FSM shows higher heat stability and higher circular symmetry of the absorption band of bacteriocholorophylls, which can be useful for the construction of new probes and reaction systems.

Genetic aspects, Membrane proteins, Photosynthesis research, Optical properties, Bacteria, Photosynthetic, Photosynthetic bacteria, Chemical properties

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Spin-polarized radical pair in photosystem I reaction center that contains different quinones and fluorenones as the secondary electron acceptor

Article Abstract:

A study was conducted to analyze the spin-polarized radical pair of the primary donor chlorophyll P700+ and the secondary acceptor Q- using the electron spin echo envelope modulation method in the photosystem I reaction center. The reaction center was characterized by reconstituted artificial quinones and fluorenones. Experimental results indicated the capability of artifical quinones to bind at the original phyQ site.

Research, Quinone, Quinones, Radicals (Chemistry)

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Direct counting of submicrometer-sized photosynthetic apparatus dispersed in medium at cryogenic temperature by confocal laser fluorescence microscopy: estimation of the number of bacteriochlorophyll c in single light-harvesting antenna complexes chlorosomes of green photosynthetic bacteria

Article Abstract:

The number of pigments in single light-harvesting complexes is calculated by imaging single chlorosomes in a frozen buffer at cryogenic temperature with a confocal laser fluorescence microscope and pigment extraction. The results have given the averaged numbers of the bacteriochlorophyll (BChl) c molecules contained in a single chlorosome of Chlorobium tepidium and Chlorobium aurantiacus.

Analysis, Fluorescence microscopy, Atomic force microscopy, Properties

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