Modeling enzyme reaction intermediates and transition states: citrate synthase

Article Abstract:

A study was conducted to analyze the functions of catalytic residues and the characteristics of the nucleophilic intermediate in citrate synthase. Computations were performed on models of the first stage of the reaction with ab initio and semiempirical techniques. Results suggested that the hydrogen bond between His-274 and the intermediate was not the low-barrier type. Findings also showed that the stabilization of the enolate supported by the hydrogen bond with the imidazole is affected by the interaction with the third component of the reacting system.

Hydrogen bonding, Hydrogen bonds, Citrates

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Salt effects on protein titration and binding

Article Abstract:

Research was conducted to examine the salt effects on binding and protein titration using a cavity-function theory for chemical association obtained with the exponential approximation. The coupling between the electrostatic interaction and hard-core interaction are investigated. Results indicate that the analytic theory more accurately predicts the electrostatic interaction factors measured in protein-titration experiments than the Debye-Huckel approximation theory.

Analysis, Proteins, Ligand binding (Biochemistry), Salts, Approximation theory, Approximation

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Electron transfer in organized assemblies of biomolecules. Step-by-step avidin/biotin construction and dynamic characteristics of a spatially ordered multilayer enzyme electrode

Article Abstract:

A study was conducted to show that avidin-biotin technology may be utilized to establish stable and spatially ordered assemblies of monomolecular enzyme layers on surfaces. The catalytic response, diffusion of the mediator and structure of the monomolecular layer assembly were analyzed using cyclic voltammetry. Results indicated that avidin-biotin technology supports the construction of stable assemblies of monomolecular layers of glucose oxidase on glassy carbon.

Surfaces (Materials), Biomolecules, Surfaces (Technology)

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