Molecular thermodynamics for protein precipitation with a polyelectrolyte

Article Abstract:

A molecular-thermodynamic model for phase equilibria was developed in an effort to assess the protein precipitation of charged globular proteins in an aqueous environment. The proposed framework makes use of charged-hard sphere chain to denote linear polyelectrolyte and a spherical macroion to represent globular proteins. Results revealed that precipitation is enhanced through the addition of polyelectrolyte into a protein solution and further addition leads to the redesolution of these compounds.

Proteins, Electrolyte solutions, Thermodynamics, Protein separation

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Lysozyme net charge and ion binding in concentrated aqueous electrolyte solutions

Article Abstract:

A study was conducted to analyze lysozyme net charge and ion bonding in electrolyte solutions of potassium chloride over the pH range 2.5-11.5 and ionic strengths to 2.0 M. A serial addition of aliquots of an alkaline titrant solution was utilized to carry out calibration titrations. Results indicated the dependency of ion-protein interactions on pH and ionic strength that is correlated with electric-double-layer theory.

Research, Ions, Electrolytes, Hydrogen-ion concentration, Potassium chloride

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Measurement of lysozyme-lysozyme interactions with quantitative affinity chromatography

Article Abstract:

The quantitative affinity chromatography is used to measure the lysozyme-lysozyme interactions at a variety of solution conditions. The interactions between lysozyme molecules are described by a potential of mean force that contains hard-sphere, electrostatic, and square-well contributions.

Chemistry, Physical and theoretical, Physical chemistry, Lysozyme, Chromatography

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