Simplified hot start PCR

Article Abstract:

Polymerase chain reaction (PCR) with thermally activated DNA polymerase gives greater product yield, and is more sensitive and specific. AmpliTaq Gold DNA polymerase used for PCR does not show any enzyme activity at room temperature but can be substituted in most PCR systems for thermostable DNA polymerase. A pre-PCR heat cycle restores the polymerase activity, and is programmable such that the conversion from enzyme activation to PCR cycling activity is above the optimal primer annealing temperature.

Analysis, Usage, Polymerase chain reaction, DNA polymerases

---

Historical highlights in therapies

Article Abstract:

Biologics have broadened the selection of drugs that might fight help in fighting autoimmunity. The new approaches for treating autoimmune diseases contain two basic elements, such as advanced therapies that attack specific components in the immune systems and developing various therapies with different mechanisms of action for same disease that benefit more from one form of therapy than another.

Methods, Drug therapy, Autoimmunity

---

The new men's movement

Article Abstract:

The Promise Keepers fundamentalist church founded by former football coach Bill McCartney has become the dominant religion in the US in the 1990s. However, feminists are concerned that the movement has aims to curtail the gains made in women's rights.

Membership, Political aspects, Feminism, Promise Keepers (Denver, Colorado), Paternalism, Fundamentalist churches

Similar abstracts:

• Abstracts: The new woman. That woman. The community speaks: one woman's complaint leads to a new park
• Abstracts: I oughta be in pictures. Home improvement. Ingenue engineering
• Abstracts: The Queen carries on. Artless philanthropy
• Abstracts: The end of Camelot. Home Depot Inc. - HD
• Abstracts: The zit list. Shaker Screen

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.