Clinical evaluation of Amplicor HIV-1 test for detection of human immunodeficiency virus type 1 proviral DNA in peripheral blood mononuclear cells
Article Abstract:
A commercially-available polymerase chain reaction (PCR) test known as Amplicor may accurately detect the presence of HIV in blood if standard blood tests cannot provide a diagnosis. Amplicor had been tested before and was found to produce too many false-negative results. Researchers used the second-generation Amplicor test to detect HIV type 1 DNA in 208 blood samples from 104 HIV-infected and 55 uninfected people. Amplicor correctly identified HIV-1 DNA in 151 of 153 blood samples from the HIV-infected patients, a sensitivity rate of 98.7%. No false-positive results were found in blood samples from the 55 uninfected people. Second-generation Amplicor was found to be improved over the first-generation in terms of sensitivity.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1995
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HIV-1-induced perturbations of glycosphingolipid metabolism are cell-specific and can be detected at early stages of HIV-1 infection
Article Abstract:
Changes to glycosphingolipids (GSL) seem to take place very early in the process of infection by HIV-1, and therefore may be an avenue for early detection. Glycosphingolipids are a type of of fatty substance formed from carbohydrates, and the changes to their metabolism are cell-specific, which has allowed labeling, followed by purification, and the radioactivity in each individual GSL examinded with phosphoimaging. This procedure seems to suggest that, if further developed, this could lead to a definitive test for early detection of the activity of HIV-1 virus.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1998
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Transmission of HIV-1 in infants born to seropositive mothers: PCR-amplified proviral DNA detected by flow cytometric analysis of immunoreactive beads
Article Abstract:
The polymerase chain reaction immunoreactive bead assay (PCR-IRB) appears to be better than some diagnostic tests for detecting HIV infection in infants. Unlike tests that detect antibodies to the virus, this test detects proviral DNA. This is necessary because babies born to HIV-infected women acquire maternal antibodies via the placenta. In a test of 50 blood samples from infants already tested by other methods, PCR-IRB produced consistent results. The test was used on 14 mother-infant pairs and it detected proviral DNA at an earlier stage than other tests.
Publication Name: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Subject: Health
ISSN: 1077-9450
Year: 1997
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