Differences in oxidative response of subpopulations of neutrophils from healthy subjects and patients with rheumatoid arthritis

Article Abstract:

There may be an association between the electrical surface charge of synovial fluid cells and inflammatory disease activity. Measuring superoxide anion levels on the cells is one way of measuring cell surface charge. Researchers isolated neutrophils from blood samples taken from 26 patients with various stages of rheumatoid arthritis (RA), synovial fluid from 3 of the patients with RA, and blood samples from healthy volunteers. They measured and compared superoxide anion production of these cell samples before and after stimulation with platelet activating factor (PAF) and various concentrations of N-formyl-methionyl-leucyl-phenylalanine (FMLP), an inflammatory mediator and stimulator, respectively. Fresh neutrophils and those stimulated with FMLP from patients with RA produced more superoxide anions than those from the healthy volunteers. The optimal concentration of FMLP that will detect differences between diseased and normal populations seems to be 10 to the -7 power moles per liter FMLP. Of the normal cells, those with low electrical charge responded the most to FMLP stimulation.

Research, Rheumatoid arthritis, Electric properties, Cells (Biology), Oxidation, Physiological, Physiological oxidation, Cells, Electrophysiology

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Human blood and synovial fluid neutrophils cultured in vitro undergo programmed cell death which is promoted by the addition of synovial fluid

Article Abstract:

The cell death or apoptosis of neutrophils may be a normal and powerful process in inflamed joint fluid. Neutrophils are white blood cells that combine with immune compounds and also digest foreign substances. Colony stimulating factors (CSFs) are lymph proteins that stimulate the development of neutrophils. Researchers tested fresh and cultured synovial fluid (SF) and blood samples from patients with various arthritic diseases for apoptotic neutrophil levels. They also tested these samples for their response to CSFs and additional SF. Apoptotic neutrophils were present in up to 22% of the fresh SF samples. However, there was no correlation between the apoptotic neutrophil concentration and disease progression. Increasing the concentrations of SF added to the samples and the exposure time to the additional SF increased the degree of neutrophil cell death. The addition of CSFs had no significant effect on decreasing the rates of neutrophil cell death.

Joints, Joints (Anatomy), Cell death, Inflammation, Synovial fluid, Colony-stimulating factors (Physiology), Colony-stimulating factors

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• Abstracts: Interleukin-1 expression by neutrophils in rheumatoid arthritis. Receptor expression in synovial fluid neutrophils from patients with rheumatoid arthritis

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