Measuring the frequency of mouse and human cytotoxic T cells by the Lysispot assay: independent regulation of cytokine secretion and short-term killing

Article Abstract:

Research demonstrates that the Lysispot assay in which cytokine-secreting and cytotoxic cells can be simultaneously enumerated is a powerful ex vivo analysis for measuring immune response during infection and autoimmunity.The assay consisting of antigen-specific, perforin-dependent Lysispot is combined with a cytokine Elispot to verify the independent regulation of cytotoxicity and interferon-gamma secretion.

Product information, Usage, Measurement, Testing, T cells, Immune response, Biological assay, Bioassay, Toxicity testing, Toxicity tests

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A quantitative assay for HIV DNA integration in vivo

Article Abstract:

This article examines techniques to help the virologist study how HIV viruses interact with DNA in vivo. A quantitative assay is described that analyzes how the virus integrates its DNA into the chromosomes of its host.

Statistical Data Included, HIV infection, HIV infections, Genetic aspects, Microbiological assay, Virologists

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A sensitive and high-throughput assay to detect low-abundance proteins in serum

Article Abstract:

A modular, facile amplification system, termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT) is created. The detection of Her2 from rodent and human sera is described.

Science & research, Research, Serum, Blood serum, Antigens

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