PCR detection of polycyclic aromatic hydrocarbon-degrading mycobacteria

Article Abstract:

Methods of polymerase chain reaction (PCR) specific for the 16S rRNA genes of Mycobacterium sp. PYR-1 and Mycobacterium sp. PAH135, both known polycyclic aromatic hydrocarbon (PAH)-degrading species, were developed. These methods are designed to detect these mycobacteria in soil slurries for use in the metabolism of PAHs. A simple and effective procedure to remove the PCR-interfering materials from soil, an improved cell lysis procedure and the introduction of direct PCR on cells without lengthy DNA purification are also described.

Analysis, Polymerase chain reaction, Mycobacteria, Mycobacterium, Polycyclic aromatic hydrocarbons

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Effect of lignin on the anaerobic decomposition of cellulose as determined through the use of a biochemical methane potential method

Article Abstract:

Biochemical methane potential experiments were conducted to evaluate the impact of lignin on cellulose decomposition. The study involved lignocellulosic components of municipal solid waste, particularly newspapers, under anaerobic conditions. Cellulose decomposition rates and total cellulose decomposition were not changed by lignin addition. Newspaper printing ink also did not affect the decomposition process.

Newspapers, Environmental aspects, Lignin

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