Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow

Article Abstract:

The temporary tethering of the selectins to the blood vessel wall leukocytes flowing in the bloodstream follows a first-order kinetics which indicates an unimolecular interaction between P-selectin and its glycoprotein ligand. The selectins slow their flow rate through the temporary tethering, with frequency dependent on the P-selectin site density. The high tensile strength of the P-selectin-to-ligand bond combined with the fast on and off bond rates and cytoskeleton anchoring allow for selectin-mediated rolling at physiological shear stresses when the leukocytes suffer hydrodynamic drag forces.

Physiological aspects, Neutrophils, Lipid membranes, Bilayer lipid membranes, Hydrodynamics

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Modifying the mechanical property and shear threshold of L-selection adhesion independently of equilibrium properties

Article Abstract:

L-selectin is expressed on leukocytes and is important in rolling interactions between leukocytes in the blood and endothelial cells of the blood vessel wall. A selective and reversible chemical version of a mucin-like ligand that can change the mechanical properties of its bond with L-selectin, is reported. The effect of force on rates of bond dissociation is changed, although there is little effect on the rate of bond dissociation without force. Also the need for hydrodynamic shear flow is much altered.

Observations, Cell adhesion molecules

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Adhesion through L-selectin requires a threshold hydrodynamic shear

Article Abstract:

Selectin-ligand bonds' high mechanical strength allows it to tether to the vessel wall through one or a few bonds as it also allows rolling in response to hydrodynamic drag. The L-selectin molecule on leukocytes tethers to the peripheral node addressin on the high endothelial venules of the lymph nodes to mediate leukocyte rolling and binds to a ligand on neutrophils to mediate rolling of leukocytes over one another. A mechanism for regulation of these interactions, in vitro and in vivo, is described.

Cell adhesion, Leukocytes, White blood cells

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