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Zoology and wildlife conservation

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Abstracts » Zoology and wildlife conservation

Max in a complex affair

Article Abstract:

A protein that fastens to a specific DNA and takes charge of the polymerization of RNA is a transcription factor called max. Proteins must distinguish and connect to definite DNA sequences after the interaction of amino acid side-chains with nucleotide bases of specific DNA sequences. The helix-loop-helix (HLH) is marked by two amphipathic alpha-helices linked by a loop. Two alpha-helices segregated by a loop is typical of a Max homodimer-DNA structure. The peculiarities of Max homodimer is how it recognizes its DNA target and the similarity between Max-DNA structure and GCN4bZIP-DNA complex.

Author: Freemont, Paul
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1993
Column, DNA, Proteins, Protein structure

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DNA-binding properties of the yeast SWI/SNF complex

Article Abstract:

A study was conducted on the binding effects of the purified yeast SWI/SNF complex on DNA. A gel retardation assay was used to test the DNA-binding properties of the yeast. Results showed that the SWI/SNF complex binds with high affinity to promoter sequences from genes that are both dependent on and independent from the complex. The apparent binding constant was estimated at 1-9 times 10(super -9)M.

Author: Peterson, Craig L., Schmidt, Martin C., Quinn, Janet, Fyrberg, Amy M., Ganster, Raymond W.
Publisher: Macmillan Publishing Ltd.
Publication Name: Nature
Subject: Zoology and wildlife conservation
ISSN: 0028-0836
Year: 1996
Genetic transcription, Transcription (Genetics), Yeast, Yeast (Food product)

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Subjects list: Research, DNA binding proteins
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