Oligomerization activates c-Raf-1 through a Ras-dependent mechanism

Article Abstract:

The oligomerization of the c-Raf-1 proto-oncoprotein by a Ras-dependent mechanism facilitates Raf activation. The addition of FK506 derivatives of FK1012A induces the oligomerization of recombinant FKBP-Raf in situ, which stimulates Raf kinase activity almost half as well as the epidermal growth factor. This activation of FKBP-Raf is dependent upon Ras-GTP, which translocates c-Raf-1 to the membrane, initiating an alteration that facilitates the interaction of the Raf polypeptide with an activator.

Physiological aspects, Enzymes, Enzyme regulation, Ras genes

---

A dimeric 14-3-3 protein is an essential cofactor for Raf kinase activity

Article Abstract:

The cRaf-1 protein kinases is mitogen-activated and is the main effector used by GTP-bound Ras to activate the MAP kinase pathway. GTP bound Ras can bind Raf but is not sufficient for the stable activation of Raf. The role of 14-3-3 proteins in the regulation of Raf's kinase activity is investigated. It is proposed that dimeric 14-3-3 is required to maintain Raf in the inactive state in the absence of GTP-bound Ras, and to stabilize active Raf.

---

A signal for Beta-cell failure

Article Abstract:

Human type 2 diabetes is usually accompanied by insulin response defects, resulting in hyperglycaemia. Withers and colleagues have reported the phenotype of mice lacking alleles encoding the insulin-receptor substrate protein-2, IRS-2. The mice develop a syndrome like the common form of human type 2 diabetes.

Diabetes, B cells, Diabetes research

Similar abstracts:

• Abstracts: NMR structure of inactivation gates from mammalian voltage-dependent potassium channels. A role for phosphatidylinositol transfer protein in secretory vesicle formation
• Abstracts: Planning rejection leaves British nuclear waste plans in disarray. Nuclear blast from the past?
• Abstracts: Clinton sketches out his 'ethical guideposts' for modern biology. Clinton woos women's votes with cancer genetics funds
• Abstracts: A rotating disk of gas and dust around a young counterpart to beta Pictoris. Stellar gerontology
• Abstracts: Fly (almost) south young bird. Resonance effects indicate a radical-pair mechanism for avian magnetic compass

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.