Orai1 is an essential pore subunit of the CRAC channel

Article Abstract:

A study is conducted to demonstrate that Orai1 is a plasma membrane protein and that [Ca.sup.2+] release-activated [Ca.sup.2+] or CRAC channel function is sensitive to mutation of two conserved acidic residues in the transmembrane segments. It was found that the E106D and E190Q substitutions in transmembrane helices 1 and 3, respectively, reduce [Ca.sup.2+] influx, increase current carried by monovalent cations, and render the channel permeable to [Cs.sup.+].

Cell membranes, Amino acids, Cytoplasm

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A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function

Article Abstract:

A novel protein Orai1 was identified which is crucial for store-operated C[a.sup.2+] entry and CRAC channel function. A modified linkage analysis with Orai1 using two unbiased genetic approaches were identified in order to identify the gene mutated in the severe combined immune deficiency (SCID) patients, and a genome-wide RNA interference (RNAi) screen in Drosophila to identify regulators of store-operated C[a.sup.2+] entry and NFAT nuclear import.

Causes of, Gene mutations, Gene mutation, Genetic research, Immunodeficiency

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A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT

Article Abstract:

A genome-wide RNA interference (RNAi) screen is used in Drosophila to identify additional regulators of the signaling pathway leading from [Ca.sup.+]-calcinerium to NAFT (nuclear factor of activated T cells). The results have shown that genetic screening in Drosophila can be successfully applied to cross evolutionary boundaries and identify new regulators of a transcription factor that is expressed only in vertebrates.

Washington, Analysis, Physiological aspects, Genetic aspects, Drosophila, Phosphorylation, RNA processing

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