Visualizing spatially correlated dynamics that directs RNA conformational transitions

Article Abstract:

The execution of the scheme, using nuclear magnetic resonance (NMR), to observe the dynamics between two RNA helices that are connected by a functionally significant trinucleotide bulge over timescales extending up to milliseconds is discussed. The 3D dynamic pathway is planned at different positions by seven separate ligand-bound conformations of the RNA and so even partly unstructured RNAs can experience structural dynamics that directs ligand-induced changes along specific predefined conformational pathways.

Technology application, RNA, Nuclear magnetic resonance, Optical properties

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Relating ligand binding to activation gating in CNGA2 channels

Article Abstract:

The fluorescent cGMP analogue 8-DY547-cGMP as the ligand was used to study the action of homotetrameric olfactory-type cyclic-nucleotide gated (CNG)A2 channels in inside-out membrane patches by simultaneously determining channel activation and ligand binding. Steady-state binding was larger than steady-state activation at concentrations of 8-DY547-cGMP<1 [mu]M whereas at higher concentrations it was smaller, generating a crossover of the steady-state relationships.

Ion channels, Cyclic guanylic acid, Cyclic guanosine monophosphate

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Open-to-closed transition in apo maltose-binding protein observed by paramagnetic NMR

Article Abstract:

The domain rearrangement in the crystal structure of apo and holo maltose-binding protein is illustrated.

Analysis, Observations, Nuclear magnetic resonance spectroscopy, Crystallography, Binding proteins

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