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Systematic mutational analysis of the yeast ACT1 gene

Article Abstract:

Systematic mutation of surface residues in yeast actin gene was analyzed by isolating and characterizing site directed mutations in the gene. The mutations were done by replacing codons for charged residues with that coding for alanine. Two mutations apparently codes for a dominant defective protein, 11 for lethal but recessive phenotypes and 16 for recessive conditional lethal traits. Seven mutations were found to code for ambiguous phenotypes. The mutant genes yields proteins readily comparable to actin.

Author: Botstein, David, Wertman, Kenneth F., Drubin, David G.
Publisher: Genetics Society of America
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
Methods, Genetic aspects, Mutation (Biology), Mutation, Saccharomyces, Actin

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The 1998 Genetics Society of America Medal: Ronald W. Davis

Article Abstract:

Ronald W. Davis is the recipient of the 1998 Genetics Society of America Medal, acknowledging his contributions to the field of modern genetics through his studies on the development of genetic technologies. Davis utilizes electron microscopes to analyze the structure of individual DNA molecules. He discovered the cohesive property of DNA ends generated by EcoRI endonuclease. In addition, Davis was instrumental in the development of bacteriophage recombinant libraries.

Publisher: Genetics Society of America
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1999
Achievements and awards, Geneticists, Davis, Ronald W.

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