A Bacillus subtilis gene cluster similar to the Escherichia coli phosphate-specific transport (pst) operon: evidence for a tandemly arranged pstB gene

Article Abstract:

The complete nucleotide sequence of the Bacillus subtilis homologues of the Escherichia coli phosphate-specific transport operon is presented as part of the international B. subtilis genome sequencing project. An operon arranged in the order pstS, pstC, pstA, pstB and phoU is formed by the pst genes in E. coli. Five ORFs are presumed to form an operon in B. subtilis. The gene corresponding to phoU is missing when the organization of the pst operon of B. subtilis was compared with that of E. coli. Two genes were also found to be homologous tp pstB in B. subtilis.

Escherichia coli, Operons

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Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes

Article Abstract:

The 283 kb fragment in the 210 degrees to 232 degrees region of Bacillus subtilis genome was cloned and sequenced to isolate the 48 kb skin element by site-specific recombinase. The region contains 310 open reading frames (ORF) with a transfer rinonucleic acid gene. The protein products of 84 ORFs mediate nucleotide metabolism, lipid biosynthesis and amino acid transport. Furthermore, analysis of the skin gene indicated the presence of 30 ORFs that encode proteins that are similar to the products of phage phi105 of Bacillus subtilis.

Identification and classification, Genetic code, Microbial genetics

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Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis

Article Abstract:

Total nucleotide sequencing of the Bacillus subtilis skin element reveals that the 48,032 bp long element has 57 open reading frames (ORFs) with putative ribosome binding sites in it. Products of seven ORFs resemble known proteins in data banks while two of them match the cwlA and spoIVCA genes that were sequenced earlier, indicating that the skin element is probably a cryptic remnant of an ancestral phage.

Binding sites (Biochemistry), Active sites (Biochemistry)

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