A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced

Article Abstract:

Examination of the three-dimensional organization of X-linked genes reveals that when transcribed, they are always located at the periphery of, or outside, the Xist RNA domain, in contact with the transcription machinery. Upon silencing, gene shifts to a more internal location, within the Xist RNA compartment devoid of transcription factors and the appearance of this compartment is not dependent on the A-repeats of the Xist transcript, which are essential for gene silencing.

United States, Genetic aspects, Nerves, Peripheral, Gene silencing, Peripheral nervous system

---

Tsix-mediated epigenetic switch of a CTCF-flanked region of the Xist promoter determines the Xist transcription program

Article Abstract:

A precisely defined Xist promoter region flanked by CTCF is maintained by Tsix in a heterochromatic-like state in undifferentiated embryonic stem (ES) cells and shifts to a pseudoeuchromatic structure upon Tsix truncation is shown. It is observed that the Tsix mediated epigenetic regulation of Xist, which resulted in Xist promoter activation, and initiation of X inactivation, in differentiating ES cells.

Chromatin, Epigenetic inheritance, Report

---

Tsix transcription across the Xist gene alters chromatin conformation without affecting Xist transcription: Implications for X-chromosomes inactivation

Article Abstract:

A demonstration that RNA polymerase II preintiation complex recruitment and H3 Lys 4 (H3-K4) methylation at the Xist promoter form the basis of the Xist expression profiles that drives both imprinted and random X-chromosome inactivation (XCI) is presented. X-chromosome inactivation (XCI) is highly dynamic during early mouse embryogenesis and strictly depends on the Xist noncoding RNA.

RNA polymerases, X chromosome

Similar abstracts:

• Abstracts: Development and evaluation of a reflective solar disinfection pouch for treatment of drinking water. Removal of astrovirus from water and sewage treatment plants, evaluated by a competitive reverse transcription-PCR
• Abstracts: SLAM family receptors distinguish hematopoie. Isolation of adult mouse myogenic progenitors: Functional heterogeneity of cells within and engrafting skeletal muscle
• Abstracts: Live-cell imaging reveals replication of individual replicons in eukaryotic replication factories. Contribution of the endoplasmic reticulum to peroxisome formation
• Abstracts: Tethering RITS to a nascent transcript initiates RNAi- and heterochromatin-dependent gene silencing. The role of the RNAi machinery in heterochromatin formation
• Abstracts: Planktonic replication is essential for biofilm formation by Legionella pneumophila in a complex medium under static and dynamic flow conditions

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.