A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores

Article Abstract:

Research was conducted to examine a new, sensitive, inexpensive and rapid bioassay for the detection of the emetic toxin of Bacillus cereus. The novel bioassay is based on the motility loss of boar spermatozoa upon 24 h of exposure to emetic B cereus strains extracts or contaminated food. Results indicate swollen mitochondria of the paralyzed spermatozoa but no epetion of cellular ATP or damage to plasma membrane integrity was observed.

Ionophores

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Bacteria, molds and toxins in water-damaged building materials

Article Abstract:

Water-damaged sites of indoor buildings are heavily colonized by Stachybotrys chartarum, Penicillium, Aspergillus, gram-negative bacteria, mycobacteria and endotoxins. Water extracts of gypsum liners from a water-damaged site show the presence of endotoxins and beta-D-glucan. The methanol extracts of water-damaged gypsum liner contain satratoxin, paralyzing the motility of boar spermatozoa at low concentrations.

Analysis, Evaluation, Building materials, Maintenance and repair, Microbial contamination

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Semiautomated metabolic staining assay for Bacillus cereus emetic toxin

Article Abstract:

A study was conducted to determine bacterial strains that facilitate emetic toxin production. Bacterial strains of Baccilus cereus, Baccillus anthracis, Bacillus licheniformis, Baccillus mycoides, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus agri, Paenibacillus macerans and Paenibacillus polymyxa were evaluated. Results indicate that only strains of Bacillus cereus exhibit cytotoxicity.

Cell-mediated cytotoxicity, Cell mediated cytotoxicity, Bacillus (Bacteria), Bacillus

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