The bacterial flagellin gene as a biomarker for detection, population genetics and epidemiological analysis

Article Abstract:

The flagellin gene is responsible for the movement of a majority of bacterial species. The arrangement of flagella in the external surface body of a bacteria, its composition, gene sequence and forms, vary to identify the type of species they inhabit upon. Microbiological researches are conducted based on these diversities, particularly with the flagellin gene's domainal structure made of N- and C- terminal regions, making them ideal candidates for the PCR amplification. Furthermore, the diversity of motile bacteria qualify for the study of population genetics for a range of organisms.

Bacteria, Microbiological research, Bacterial motility

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Comparison of flagellin genes from clinical and environmental Pseudomonas aeruginosa isolates

Article Abstract:

The isolation of Pseudomonas aeruginosa strains obtained from an experimental mushroom-growing unit and other sources along with the analysis of the flagellin gene sequences have been undertaken using polymerase chain reaction restriction fragment length polymorphism analysis. P. aeruginosa is the most common pathogen associated with cystic fibrosis and is a major cause of hospital infections especially in immunocompromised patients. Results indicate that it is difficult to distinguish the environmental from the clinical types of P. aeruginosa.

Bacterial genetics, Bacterial proteins

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Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa

Article Abstract:

The flagellin gene is a highly useful genetic marker with many applications for studying genetic variation in closely related bacteria. PCR amplification and RFLP analysis of flagellin gene sequences from 64 clinical isolates of Pseudomonas aeruginosa comprised a strategy for differentiating between clinical isolates of P. aeruginosa. The technique proved to be quick, reproducible and applicable to all isolates.

Biological diversity, Biodiversity, Flagella (Microbiology), Flagella

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