Interchromosomal transfer of epigenetic states in Ascobolus: transfer of DNA methylation is mechanistically related to homologous recombination

Article Abstract:

The interaction between methylation transfer and meiotic recombination is addressed by a statistical approach. Evidence of methylation transfer in conjunction with meiosis is provided by crosses between methylated and unmethylated strains of the corresponding b2 alleles. Both methylation transfer and gene conversion show the same 5'-to-3' polarity along the b2 gene. Transfer of methylation also accompanies the conversion of a mutated site in the recipient unmethylated b2 allele. Both reactions are hindered by ectopic positioning of two b2 alleles or by nucleotidic divergence.

Physiological aspects

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Isolation of the Ascobolus immersus spore color gene b2 and study in single cells of gene silencing by methylation induced premeiotically

Article Abstract:

The mechanism of an epigenetic process called methylation induced premeiotically (MIP) in Ascobolus immersus was investigated in cloned A. immersus spore color gene b2, a site of active meiotic recombination. Several DNA transformation procedures were performed to isolate the whole gene and to produce a null b2 allele. Application of MIP to a duplicate b2 confirmed gene silencing after meiosis in single cells. Results also support that MIP regulates b2 expression.

Fungi, Genetic transformation

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Suppression of crossing-over by DNA methylation in Ascobolus

Article Abstract:

DNA methylation appears to disrupt the meiotic recombination of the Ascobolus immersus fungus. Genome integrity is preserved because of this suppression as it stops chromosome rearrangements. When b2 spore color gene was methylated the crossing-over between the two markets next to the gene was considerably reduced.

Influence, Crossing over (Genetics)

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