Gene transfer of Alcaligenes eutrophus JMP134 plasmid pJP4 to indigenous soil recipients

Article Abstract:

Alcaligenes eutrophus JMP134 transfers its pJP4 catabolic plasmid, containing gene coding for degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and mercury resistance, to indigenous bacteria present in soils. The inoculation of 2,4-D-enriched soil with A. eutrophus produces a large number of transconjugates that possess mercury resistance and 2,4-D degradation ability. The transconjugates are Pseudomonas glathei, Burkholderia caryophyllii and B. cepacia strains. No bacteria with mercury resistance and 2,4-D degradation ability are present in uninoculated soil.

Genetic aspects, Plasmids

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Degradation of chlorophenols by Alcaligenes eutrophus JMP134(pJP4) in bleached kraft mill effluent

Article Abstract:

Alcaligenes eutrophus JMP134(pJP4) degrades 2,4-dichlorophenoxyacetate (2,4-D), 2,4,6-trichlorophenol (2,4,6-TCP), 2,4,5-TCP and 4-chlorophenol present in bleached kraft mill effluent (BKME). The indigenous bacteria degrades 70 to 100% of the 2,4-D and 2,4,6-TCP after six days of incubation. High bacterial and chlorophenol levels in short-term cultures of BKME suppress the growth and metabolism of A. eutrophus. The acclimation of the bacteria in long-term semicontinuous cultures allows the maintenance of a stable bacterial population and 2,4,6-TCP degradation.

Bacteriology, Chlorophenols, Bacterial cultures

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Constitutive expression of the cloned phenol hydroxylase gene(s) from Alcaligenes eutrophus JMP134 and concomitant trichloroethylene oxidation

Article Abstract:

The expression of Alcaligenes eutrophus genes encoding phenol hydroxylases influences trichloroethylene (TCE) oxidation under limited carbon conditions and the absence of aromatic induction. Ethanol decreases phenyl hydroxylase activity, while benzoate stimulates catechol 1,2-dioxygenase activity. This suggests that phenol metabolism is involved in TCE degradation. The region encoding phenol hydroxylase has significant homology to the Pseudomonas pickettii oxidoreductase and oxygenase subunits of toluene-3-monooxygenase.

Physiological aspects, Microbial metabolism, Gene expression, Trichloroethylene

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